159-188 ABSTRACTS.indd

s of oral and poster presentations given at the 6th International Workshop on Grapevine Trunk Diseases, Florence, Italy, 1–3 September 2008 The 6th International Workshop on Grapevine Trunk Diseases, was held in Florence, Italy, on September 1–3, 2008, and was attended by 120 participants. Thirty-four oral presentations and 59 posters were presented at the meeting, dealing with esca and other trunk diseases of grapevine. Some of these presentations were then completed, submitted and peer-reviewed as original research work, and they are published in full in this issue as full papers or short notes. The abstracts of the remaining presentations are also published here. PATHOGEN IDENTIFICATION AND DETECTION Phenotypic and genetic analysis of Phaeomoniella chlamydospora isolates from grapevines in Spain. M.L. TELLO, D. GRAMAJE, A. GÓMEZ, P. ABADCAMPOS 2 and J. ARMENGOL. Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), Autovía A-2, Km. 38,200, 28800 Alcalá de Henares, Spain. Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain. E-mail: [email protected] Phaeomoniella chlamydospora is the main fungal pathogen involved in Petri disease of young grapevines, causing severe losses all over the world. However, up to now, there were no previous studies related to the phenotypic and molecular diversity of this fungus in Spain, which constituted the main objective of this work. For this study, 57 isolates of Pa. chlamydospora were selected. They were obtained from symptomatic vines, between 2001 and 2005, and from a wide range of very diverse Spanish viticulture areas and rootstock/ scion combinations. Three reference Pa. chlamydospora isolates (CBS 117179, CBS 23974 and CBS 101571) and one Pa. zymoides (CBS 121168) were included. For their phenotypical characterization, Phoma-like synanamorph production, sporulation and mycelial growth rates at 5, 10, 15, 20, 25, 30 and 35°C were studied. Molecular characterization by means of DNA markers was performed with the analysis of 5 ISSR and 24 RAPD markers. This study allowed us to determine the cardinal temperatures of the Spanish Pa. chlamydospora isolates and of the P. zymoides isolate. Our results showed several differences from previously published work with isolates from other countries. Synanamorph formation was confi rmed on all isolates. Both phenotypical and molecular data showed strong homogeneity among all Spanish Pa. chlamydospora isolates, regardless of their geographical origin, year of isolation and rootstock/scion combination. Acknowledgements: This study was supported by the projects RTA2007-00023-C04-03 and RTA2007-0023-C04-04 fi nanced by the Spanish Ministry of Education and Science (“Programa Nacional de Recursos y Tecnologías Agrarias”) and the European Union (FEDER program). Phytopathol. Mediterr. (2009) 48, 159–188